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immunomagnetically purified cd4+ t cells  (STEMCELL Technologies Inc)

 
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    STEMCELL Technologies Inc immunomagnetically purified cd4+ t cells
    Immunomagnetically Purified Cd4+ T Cells, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/immunomagnetically+purified+cd4+++t+cells/immunomagnetically+purified+splenic+cd3++t+cells/pm34962571-53-23-21
    Average 90 stars, based on 1 article reviews
    immunomagnetically purified cd4+ t cells - by Bioz Stars, 2026-09
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    immunomagnetically purified cd4+ t cells  (STEMCELL Technologies Inc)
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    STEMCELL Technologies Inc immunomagnetically purified cd4+ t cells
    Immunomagnetically Purified Cd4+ T Cells, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/immunomagnetically+purified+cd4+++t+cells/immunomagnetically+purified+splenic+cd3++t+cells/pm34962571-53-23-21
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    immunomagnetically purified cd4 + t cells  (STEMCELL Technologies Inc)
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    STEMCELL Technologies Inc immunomagnetically purified cd4 + t cells
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    Quantification of gene expression patterns of inflammatory and osteoclastogenic cytokines in purified CD4+ T cells in mice at 20 months of age. CD4+ T cells were immunomagnetically purified from spleens of 20-month-old wild-type (WT) C57BL6/J and T-cell-reconstituted TCRβ knockout (KO) mice (T cells) and subjected to NanoString mRNA gene quantification for common osteoclastogenic and inflammatory cytokines: (A) mRNA for downstream osteoclastogenic mediators (RANKL, osteoprotegerin, and the RANKL/osteoprotegerin ratio). (B) Inflammatory mediators of osteoclastogenesis (tumor necrosis factor [TNF]α and IL-1β) and (C) IL-17A and IL-6. Data are expressed as boxplots (median ± interquartile range) with 10 to 90 percentile whiskers: ∗P < .05 and ∗∗P < .01 by Mann-Whitney U test. Nonsignificant comparisons not shown. n = 5 WT and 7 T-cell mice/group.

    Journal: The Journal of Infectious Diseases

    Article Title: Immune Reconstitution Bone Loss Exacerbates Bone Degeneration Due to Natural Aging in a Mouse Model

    doi: 10.1093/infdis/jiab631

    Figure Lengend Snippet: Quantification of gene expression patterns of inflammatory and osteoclastogenic cytokines in purified CD4+ T cells in mice at 20 months of age. CD4+ T cells were immunomagnetically purified from spleens of 20-month-old wild-type (WT) C57BL6/J and T-cell-reconstituted TCRβ knockout (KO) mice (T cells) and subjected to NanoString mRNA gene quantification for common osteoclastogenic and inflammatory cytokines: (A) mRNA for downstream osteoclastogenic mediators (RANKL, osteoprotegerin, and the RANKL/osteoprotegerin ratio). (B) Inflammatory mediators of osteoclastogenesis (tumor necrosis factor [TNF]α and IL-1β) and (C) IL-17A and IL-6. Data are expressed as boxplots (median ± interquartile range) with 10 to 90 percentile whiskers: ∗P < .05 and ∗∗P < .01 by Mann-Whitney U test. Nonsignificant comparisons not shown. n = 5 WT and 7 T-cell mice/group.

    Article Snippet: Expression of RANKL, OPG, TNFα, interleuking (IL)-1β, IL-17A, and IL-6 messenger ribonucleic acid (mRNA) was quantified from 50 000 immunomagnetically purified (StemCell Technologies) CD4 + T cells lysed directly in 1:3 RLT buffer (QIAGEN, Germantown, MD) and hybridized with gene-specific fluorescent barcoded probes at 65°C for 24 hours without RNA extraction.

    Techniques: Gene Expression, Purification, Knock-Out, MANN-WHITNEY